Functional expression of green fluorescent protein derivatives in Halobacterium salinarum
نویسندگان
چکیده
منابع مشابه
Analysis of Protein Phosphorylation in Halobacterium salinarum
Protein phosphorylation plays an important role in the two-component system based signal transduction pathways in bacteria and in archaea. These cascades have been identified also in the extremely halophilic archaeon Halobacterium salinarum and are well understood. Beside these protein phosphorylation events in which phosphorylation of histidine and aspartate residues are crucial, only little i...
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Halobacterium is one of the few known Archaea that tolerates high levels of sunlight in its natural environment. Photoreactivation is probably its most important strategy for surviving UV irradiation and we have shown that both of the major UV photoproducts, cyclobutane pyrimidine dimers (CPDs) and (6-4) photoproducts, can be very efficiently repaired by photoreactivation in this organism. Ther...
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The identification of 114 integral membrane proteins from Halobacterium salinarum was achieved using liquid chromatography/tandem mass spectrometric (LC/MS/MS) techniques, representing 20% of the predicted alpha-helical transmembrane proteins of the genome. For this experiment, a membrane preparation with only minor contamination by soluble proteins was prepared. From this membrane preparation ...
متن کاملBacterioopsin-mediated regulation of bacterioruberin biosynthesis in Halobacterium salinarum.
Integral membrane protein complexes consisting of proteins and small molecules that act as cofactors have important functions in all organisms. To form functional complexes, cofactor biosynthesis must be coordinated with the production of corresponding apoproteins. To examine this coordination, we study bacteriorhodopsin (BR), a light-induced proton pump in the halophilic archaeon Halobacterium...
متن کاملMicroarray Analysis in the Archaeon Halobacterium salinarum Strain R1
BACKGROUND Phototrophy of the extremely halophilic archaeon Halobacterium salinarum was explored for decades. The research was mainly focused on the expression of bacteriorhodopsin and its functional properties. In contrast, less is known about genome wide transcriptional changes and their impact on the physiological adaptation to phototrophy. The tool of choice to record transcriptional profil...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1998
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(98)00403-0